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目的了解尉犁县人间布病感染情况。Objective To find out the infection of brucella among local people.

布氏杆菌是人畜共患的重要病原之一。Brucella is one of the main pathogens to both human being and animals.

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方法用血清学试验方法检测人群布鲁氏菌抗体，了解布病感染情况。Methods Serological tests were made to detect brucella antibodies in the population.

布鲁氏菌病加强部门协作，搞好地区联防。The Brucella strengthens the department cooperation, does well the local joint defense.

目的探索一种布鲁菌分型的快速、准确、可靠的分子生物学方法。Objective To explore a rapid, accurate and reliable procedure for molecular typing of Brucella.

方法采用血清学及流行病学调查方法，对重点人群及牲畜进行布氏菌病检测。Methods To adopted the methods of serology an epidemiology and detect brucella in the crowd and livestock.

本方法同样可用于血液或其他流产病料中布鲁氏菌DNA的检测。The method can also be used for the examination of Brucella DNA in serum or other aborted pathologic materials.

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2009年对18例布病患者进行病原菌血培养，有2株为布鲁氏菌羊种菌3型。Blood culture of the pathogens collected from 18 patients in 2009 revealed 2 strains of type 3 Brucella melitensis.

寻找合适的布鲁氏菌诊断性抗原和研制具有标记性的疫苗一直是人们追求的目标。Development of a marker of the vaccine and find a suitable brucella diagnostic antigen has been the people's goals.

本发明公开了一种流产布鲁氏菌疫苗株S19标记重组菌及其应用。The invention discloses an abortus Brucella vaccine strain S19 marked recombinant strain and an application thereof.

本发明公开了一种流产布鲁氏菌重组菌及其在制备疫苗中的应用。The invention discloses an abortus Brucella vaccine recombinant strain and an application thereof in preparing vaccine.

布鲁菌病是由布鲁菌属的细菌侵入机体引起传染-变态反应性的人畜共患传染病。Brucellosis is an allergy anthropozoonosis caused by Brucella infection Medicine, and widely prevalent all over the world.

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结论制备、克隆了布鲁氏菌外膜蛋白Omp25基因片段，并在大肠杆菌中表达出Omp25融合蛋白。Conclusion The OMP25 gene of Brucella are cloned and the recombinant OMP25 protein are successfully expressed in E. coli HB101.

目的研究人用皮内注射布氏菌活疫苗的毒理及药效学作用，以评价其可行性。Objective To study the toxicology and efficacy and evaluate the feasibility of live Brucella vaccine for intradermal injection.

本发明公开了一种流产布鲁氏菌免疫标记重组菌株与其在制备疫苗中的应用。The invention discloses brucella abortus immune marking recombinant strain and the application thereof in preparation of vaccine.

本发明公开了一种羊种布鲁氏菌免疫标记重组菌株及其应用。The invention discloses a brucella melitensis immune labeled recombinant strain and the application thereof in the preparation of vaccine.

结论PFGE方法可用于布鲁菌的基因分型的研究，是对传统分型方法一种较好的补充。Conclusions PFGE can be used to classify and identify Brucella and it is a useful supplementary method for traditional typing method of Brucella.

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布鲁氏菌是兼性胞内寄生菌，它们主要定居在巨噬细胞和胎盘滋养层细胞这两类宿主细胞，。Brucella spp. are facultative intracellular pathogen that resides predominantly within two host cell types, macrophage and placental trophoblasts.

布鲁氏菌病鉴别诊断主要与伤寒、副伤寒、风湿热、肺结核、疟疾等相鉴别。The Brucella differential diagnosis mainly with typhus, the paratyphoid, rheumatic fever, pulmonary tuberculosis, malaria and so on distinguishes.

血培养是诊断布氏菌病的金标准，血清凝集试验是快速有效的辅助诊断方法。Brucella blood culture is considered as the gold standard for the diagnosis of brucellosis and Brucella agglutination test is fast and effective as an assistant diagnostic method.