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该菌株产酶发酵条件的研究,为褐藻胶裂解酶的大规模制备及应用奠定了基础。All of this lay a solid foundation for large-scale preparation and application of alginate lyase.
随着果胶裂解酶的新用途的不断出现,因此该酶是近年国内外研究的热点课题。As new use of pectate lyase is continuously found, the enzyme has been a hot spot in scientific study.
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鉴于此,我们希望该研究工作能够为日后该类蛋白的结构和功能的进一步研究提供一点帮助。This work provides important support for further research of the structure and functions of pectate lyase.
然后通过体内重组检测裂合酶活性的变化,研究色氨酸残基的突变对裂合酶催化活性的影响。The lyase activity of these mutants were tested by reconstitution in vivo to study the effects of tryptophan to CpeS.
我们首次描述传染性病猪病原即猪链球菌进行了透明质酸裂解酶的鉴定和纯化。We describe, for the first time, identification and purification of hyaluronate lyase from the zoonotic pig pathogen Streptococcus suis.
苯丙氨酸解氨酶的株间差异相对较小,但在总体水平上,抗病无性系的酶活性和比活性也比易感株略高。Phenylalanine ammonia lyase activities of the resistant clone needles, in general, were also a bit higher than those of susceptible individuals.
该结果说明,柠檬酸裂解反应决定着红发夫酵母细胞质中乙酸辅酶A的供给,从而显著影响着其虾青素的合成。These results showed that ATP, citrate lyase reaction determines the supply of acetyl-CoA, the precursor for astaxanthin biosynthesis, in cytosol of this yeast.
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近年来又发现了果胶酸裂解酶还可用于植物病毒的纯化、纸浆漂白和以纺织品的生物精炼等。It was also found recently that the pectate lyase could be used for the purification of plant virus, bleaching of paper pulp and the bio-refining of textiles etc.
苹果酸的添加降低了异柠檬酸裂解酶的活性和L-谷氨酸的产量,残糖量和细胞生长没有明显的规律性变化。The addition of malic acid decreased the activity of isocitrate lyase and the output of L-glutamate and the residual sugar content and cell growth had no obvious regular change.
以甲酚红作指示剂,在加有酪氨酸的固态培养基上,根据甲酚红由黄变红所形成的变色圈的大小筛选酪氨酸酚解酶高产突变株是一种平板快速筛选方法。It is a rapid method for screening the tyrosine phenol lyase producer according to the size of the red color ring by using methyl phenol red as the indicator in the solid medium containing tyrosine.