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他说“你可以将基因转染进成纤维细胞”。"You can transfect a gene into a fibroblast, " he said.

将重组质粒转染293A细胞，免疫印迹鉴定目的基因的表达情况。Transfect the recombination DNA into 293A and identify the gene expression.

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我们还合成了磷酸钙-四氧化三铁复合物，应用于基因的可控运输和转染。Then we prepared calcium phosphate-magnetite hybrid materials to deliver and transfect DNA.

硕士期间，参与863课题的研究，进行白细胞介素-2转染肿瘤细胞的基础研究。Master period, I participated in 863 project, carries on IL- 2 transfect to tumour cell's basic research.

基因治疗是将DNA转染进入目的细胞，修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。The aim of gene therapy is to transfect DNA into target cells, in which utilizing antisense oligonucleotides is a main method.

近年来，使用体外分析、叶绿体转化和紫外交联等技术，使叶绿体RNA编辑机制的研究取得较大进展。So far, RNA editing has made rapid progress by using in vitro editing system, UV cross-linking assays and chloroplast transfect technology.

结果合成的DNA转运系统和经修饰的病毒载体体外转染靶细胞后，可获得显著表达。Results Artificial synthetic DNA transfer system and modified viral vectors could efficiently transfect target cells and get high level expression.

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本研究认为鱼精蛋白改性磷酸钙是一种比共沉淀法合成磷酸钙更好的转染载体。It could be expected that nucleoprotamine modified calcium phosphate could be a better transfect agent as compared to coprecipitated superphosphate.

从技术应用远景来看，他们导入雌激素受体的技术对于研究大脑中甾体激素受体功能是一大进步。From a technology perspective, their technique to transfect the estrogen receptor is an exciting advance for researching steroid receptors in the brain.

目的构建人CCR7基因真核表达质粒，使其在真核细胞中稳定表达，为其临床应用奠定基础。To construct a eukaryotic expression vector of human CCR7 gene and transfect stably it into A549 cells, so to set foundation for its clinical application.

如何将肿瘤治疗基因安全高效的转移到人体内，使治疗基因在人体内长期、稳定、高效的表达是当前基因治疗实践中所遇到的重大困难。The main difficulty in its practice is how to make the therapeutic genes transfect into the human body efficiently and safely and express endurably, stably and efficiently.

本研究采用体外合成的全长HCV感染性克隆转染树鼩，观察HCV感染性克隆体内转染树鼩后病毒转归情况，探讨HCV感染树鼩动物模型的可能性。So we think tupaia is a potential animal model of HCV susceptibility. In this study we use the full-length HCV infectious clone that synthesized in vitro to transfect tupaia in vivo.