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PCR产物经限制性内切酶消化、序列测定及分析。PCR products were digested with endonuclease and sequenced.

通过核酸内切酶酶切分析及测序鉴定载体结构。The structure of vector was identified by endonuclease analyzing and base sequencing.

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经限制性内切酶反应，获得两个预期大小的片段。After a restriction endonuclease cleavage, two expected smaller fragments were observed.

TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。The core technology in TILLING is screening mutant library by using single-strand endonuclease.

采用酶切分析及序列测定等方法鉴定重组质粒的正确性。The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.

本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.

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结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.

结果经PCR方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

有13个样品在CP4-EPSPS基因检测中虽扩增出类似阳性对照的条带，但对PCR产物的进一步酶切鉴定表明，所有扩增结果为假阳性。CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.