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胰岛素原在粗糙型内质网中合成。Proinsulin is formed in rough endoplasmic reticulum.

胰岛素原在粗糙型内质网中合成。Proinsulin is formed on rough endoplasmic reticulum.

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胰岛素原水平是胰岛素抵抗的独立相关因素。Proinsulin was an independent affective factor of insulin resistance.

同时，也用PAM树脂合成了人胰岛素原C肽类似物。The preparation human proinsulin C-peptide of analogue using of PAM resin was also reported.

合成了胰岛素原C肽三聚体的基因，并在大肠杆菌中得到高效表达。A gene fragment encoding three copies of proinsulin C peptide was synthesized and expressed in E.

对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.

MBHA树脂相对价格便宜，此方法是制备人胰岛素原C肽的另一种尝试。MBHA resin is relatively inexpensive. The new method is another way for the preparation of human proinsulin C-peptide.

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所构建的重组人胰岛素原基因能指导靶细胞随葡萄糖浓度的变化调节胰岛素分泌。The cells combined human proinsulin gene has the ability of producing insulin with increase in glucose concentration in vitro.

用表达载体YTI-15转化酵母63号株可得到每升约2毫克的人胰岛素原分泌产物。Secreted proinsulin amounted to about 2 mg per liter was found in the culture medium of yeast strain 63 transformed by plasmid YTI-15.

结果人胰岛素原基因的B10位组氨酸编码序列CAC突变为门冬氨酸编码序列GAC。Results The sequence of CAC coding for Histidine at B10 site of human proinsulin gene was mutated to the sequence of GAC coding for AsparticAcid.

将胰岛素原基因融合到金色葡萄球菌蛋白A的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein A.

肝癌细胞内不含有PC2和PC3，不能有效地加工胰岛素原，故不能分泌成熟的胰岛素。In normal individual, conversion of proinsulin to insulin is mediated by two endoproteases, PC2 and PC3. There are no PC2 and PC3 but furin in hepatoma cells.