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卵黄抗体热稳定性检测。Detection of IgY thermal stability.

经免疫印迹分析，具有良好的免疫学特异性。Western blot showed the good immunological specificity of the IgY.

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目的获得鸡卵黄抗体，并研究该抗体在鸡的黄液中表达的进程。Objective To obtain IgY and study on the expression of it in egg yolk.

抗体于4℃保存十个月，活性没有明显变化。Stored in 4℃ for 10 months, the activity of IgY could not change obviously.

用制得的抗内毒素卵黄体对小鼠进行动物毒性试验及动物保护试验。The IgY was used in the experiments of mice to test the toxity and the protective effect to it.

两种抗体均可耐受胃蛋白酶作用，但对胰蛋白酶敏感。The two kinds of IgY could resist to the enzymolysis of pepsin, but were sensitivity to the trypsin.

为研究仔猪血液内复方中药免疫球蛋白的动态变化提供了可靠的检测手段。At first , it could be a based method for studying the dynamics changes of compound herbal IgY in new-born piglet.

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未发现抗内毒素卵黄抗体对小鼠的毒性作用，小鼠保护试验结果表明，抗内毒素卵黄抗体对小鼠腹泻和死亡模型具有明显的保护作用。The toxity to mice of IgY was not found. 4. The passive protective experiment showed that IgY has evident protective effect to the mice diarrhea and death model.

该蛋白已广泛应用于兽医学、功能食品和生物制品等方面，且在疾病预防控制和治疗方面开发潜力广阔。With these advantages, IgY has been widely used in veterinary science, functional food and bio-products, and proved potential application for disease control and treatment.

纯化的卵黄抗体分别置于6个EP管中，1～4号管的卵黄抗体仍有活性，第5管和第6管出现白色沉淀，可能是较高温度下蛋白变性引起的。Purified IgY was added to six EP tubes, the number 1-4 still had partial activity, but number 5 and 6 showed some white precipitate, which was caused by protein denaturation at higher temperature.