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方法免疫细胞化学和原位杂交技术。Methods Immunocytochemistry and in situ hybridization.

以免疫细胞化学法检测BACE基因的表达情况。The expression of the BACE gene was detected by immunocytochemistry.

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免疫细胞化学检测细胞表面抗原。The antigen on the cell surface was detected with immunocytochemistry.

其中8例另行胶体金标记免疫电镜观察。Colloidal gold double labeled immunocytochemistry was done in 8 patients.

方法神经干细胞培养，免疫细胞化学及RT-PCR技术。Methods Neural stem cell culture, Immunocytochemistry and RT-PCR technique were used.

免疫细胞化学鉴定出神经干细胞、神经元和胶质细胞。The nerve stem cells, neurons and glial cells were identified by immunocytochemistry.

免疫细胞化学染色显示附着于丝素上的细胞为神经元。The stretched cells on the silk fibroin are neurons that were identified by immunocytochemistry.

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免疫细胞化学证实，该模型具有BIU-87细胞的特征。Immunocytochemistry confirmed that the tumor model maintained the biological and immunological features of BIU-87 cells.

TPO免疫化学染色可用于甲状腺针吸细胞，作为诊断甲状腺良、恶性肿瘤的辅助手段。FNA samples can be used for TPO immunocytochemistry staining as an adjuvant tool for distinguishing benign and malignant tumors.

收集培养至第3周的悬浮细胞，采用免疫细胞化学法进行CD34单克隆抗体鉴定。The suspended cells were collected after 3 weeks of culture for anti-CD34 monoclonal antibodies identification by immunocytochemistry.

利用免疫细胞化学方法在激光共聚焦扫描显微镜下观察该细胞表面抗原的表达情况，进行鉴定。To observe the cell surface antigen of rat EHSCs with the confocal laser scanning microscope, using immunocytochemistry for identification.

方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.

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本文用HRP逆行追踪与免疫细胞化学结合法研究了大白鼠小脑皮质内脑啡肽能神经纤维的来源。The origin of enkephalinergic nerve fibers in the cerebellar cortex was studied by combined method of retrograde HRP tracing and immunocytochemistry.

免疫细胞化学检测不同浓度顺铂干预前后IGF-1R蛋白表达差异。Test the differences of IGF-1R protein expression before and after the intervention of cisplatin with different concentrations through immunocytochemistry.

采用降钙素基因相关的缩氨酸和神经丝蛋白双重免疫细胞化学染色法鉴定并测定神经元的纯度。Immunocytochemistry was performed by double staining with anti-calcitonin gene-related peptide and anti-NF200 antibodies to evaluate the purification rate of DRG neurons.

采用免疫荧光染色检测GFAP阳性神经前体细胞系移植到动物体内后神经元特异性标志物表达情况。Immunocytochemistry was used to examine the expression of the neuronal specific marker after transplantation the GFAP positive neural progenitor cell line into the animal model.

本实验用HRP逆行追踪和免疫细胞化学结合法研究了大鼠脑桥内GABA能神经元至小脑前叶皮质的投射。Projections from GABA-ergic neurons of the pons to the cortex of anterior cerebellar lobe have been studied by combined method of HRP retrograde tracing and immunocytochemistry.

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本文用免疫细胞化学和HRP结合法研究了大白鼠躯体感觉神经节内含P物质的神经元、含生长抑素的神经元及其投射。Substance P-containing neurons and Somatostatin-containing neurons and their projections were studied in the somatic sensory ganglia of rats with combined method of HRP and Immunocytochemistry.

用免疫细胞化学方法，观察慢性浅表性胃炎脾胃湿热证舌苔脱落细胞CDK4和CDK6的表达的意义。To probe the meaning of coated tongue cast-off cells CDK4 and CDK6 expression in the patients of chronic superficial gastritis with splenogastric hygropyrexia type by immunocytochemistry method.

方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.