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本发明公开了硝基呋喃类物质的一种半抗原、抗原及其制备方法与应用。The invention discloses hapten and antigen of nitrofurans and a preparation method and application thereof.
本发明公开了一种苏丹红半抗原与抗原及其制备方法与应用。The invention discloses melamine hapten and antigen as well as a preparation method and application thereof.
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免疫半抗原的分子设计与合成是建立小分子免疫化学分析方法的关键步骤。The hapten design and synthesis are the key steps for the development immunochemical method of pesiticide residue.
本发明公开了一种呋喃唑酮代谢物半抗原、抗原及其制备方法和应用。The invention discloses furazolidone metabolite hapten and antigen as well as a preparation method and application thereof.
半抗原免疫学检测方法具有较低的检测极限、快速、方便、低廉等优势,是食品安全检测的发展方向。Hapten immunoassay methods for food safety detection have the advantages of low detection limit, fast detection, convenience and low cost.
在半抗原成形后,他们将这些新的半抗原分子附到蛋白质载体上并与辅助剂混合再注射到小鼠身上等待结果。Once built, they attached the new hapten molecules to carrier proteins, mixed them with adjuvant, injected the results into mice and waited.
实验中合成了两种不同碳桥长度的苏丹红衍生物,并将该衍生物与载体蛋白质交联。Two hapten derivatives with different length of carboxylic spacer at the azobound para-position were synthesized and coupled to carrier proteins.
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修饰的乙肝核心蛋白或其聚集的核衣壳蛋白颗粒可悬垂连接到半抗原上以形成一修饰的核衣壳偶联物。The modified hepatitis B core protein or its aggregated nucleocapsid protein particles can be pendently linked to a hapten to form a modified nucleocapsid conjugate.
我们的进一步研究还发现,即便半抗原没有被修饰,这种遗迹仍然能够作为一个信号,诱导可识别载体蛋白的结合物的演变。We further show that such an imprint can act as a seed in the evolution of binders that recognize the carrier protein even in the absence of the hapten modification.
酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用,其灵敏度取决于标记酶的高度纯化和高转化率。The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label.
并对拟除虫菊酯类农药通用免疫半抗原设计及多残留酶免疫分析技术的发展趋势进行了探讨。The development of enzyme immunoassays for the determination of pyrethroid residues were reviewed, including hapten design, antibody preparation, and immunoassay establishment and applications.