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人源性天然抗体库的构建。The construction of phage library of scFV antibody.

噬菌体已复制和给体细胞已发生裂解。The phage has replicated and the donor cells have lysed.

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通过三轮生物淘洗过程，噬菌体克隆得到富集。After three rounds biopanning, the phage clones have enriched.

它能在较长时间内稳定生产噬菌体抗体。It was possible to produce phage antibody steadily in long time.

用ELISA和流式细胞仪检测展示在噬菌体上CD28的抗原性。The antigenicity of CD28 displayed on phage was detected by ELISA and FCM.

每当菲姬对任一牌手造成战斗伤害时，该牌手输掉这盘游戏。Whenever Phage deals combat damage to a player, that player loses the game.

该分型方案为菌株的噬菌体分型提供了模式。This typing scheme has provided the patterns for the phage typing of strains.

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噬菌体抗体库是近年发展起来的一项分子生物学新技术。Phage antibody library is a new technology of Molecule Biology in recent years.

M13噬菌体已被广泛地应用于噬菌体展示技术。M13 bacteriophage has been extensively applied in phage surface display technique.

本文仅就噬菌体展示技术及其在感染性疾病中的应用作一综述。Phage display and its application to infectious diseases are reviewed in this paper.

伴随着溶源菌与非溶源菌接合的噬菌体诱导。Phage induction following conjugation of a lysogenic bacterium with a nonlysogenic one.

目的建立一种测定噬菌体抗体相对亲和力的方法。Objective To establish a method for the measurement of relative affinity of phage antibodies.

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侵染后约30分钟，细菌细胞裂解，释放出噬菌体颗粒。About 30 minutes after infection the bacterial cell lyses and the phage particles are released.

结论硫氰酸盐洗脱法可用于噬菌体抗体相对亲和力的测定。Conclusion The relative affinity of phage antibodies could be determined by ELISA using thiocyanate.

本研究建立了一套膜生物反应器系统，并首次用于噬菌体抗体的生产。Here a membrane bioreactor system was set up, and was used to produce phage antibody for the first time.

噬菌体展示及筛选和DNA重排是体外定向分子进化的重要核心技术。Phage display and DNA shuffling are important and efficient techniques for directed molecular evolution.

DNA重排和噬菌体展示及筛选是体外定向分子进化的重要核心技术。Phage display and DNA shuffling are important and efficient techniques for directed molecular evolution.

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经过三轮筛选，随机挑取48个噬菌体单克隆，进行ELISA分析。After 3 rounds of panning, 48 single phage plaques were randomly picked and ELISA analysis was performed.

目的从噬菌体12肽库中筛选卫氏并殖吸虫抗原模拟表位。Objective To screen the mimic antigen epitope of Paragonimus westermani from phage 12-mer peptide library.

随着此项技术的不断完善和发展，噬菌体肽库已在许多领域得到了广泛的运用。With the development of phage display libraries, it might be applied extensively in many fields of research.