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对甘薯胚性细胞悬浮培养系的建立进行了研究。Establishment of embryogenic cell suspension cultures in sweet potato was studied.

长期的饥饿和衰老可以导致胚胎发生能力的复壮。Prolonged starvation and aging might lead to rejuvenation of embryogenic potential.

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胚性悬浮细胞是禾谷类作物原生质体培养的理想材料。Embryogenic suspension cell is ideal material of protoplast culture of gramineous plants.

结果表明，胚性愈伤组织是棉花染色体观察的最佳材料。The results indicated that embryogenic callus was the best material for observed chromosome.

从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice.

基因型和2，4-D浓度对愈伤组织状态调节，至为关键。Genotypes and concentration of 2,4-D was critical to induce embryogenic and somatic embryogenesis.

MSB培养基中添加IBA和BA也不能直接诱导获得胚性愈伤组织。Embryogenic callus could be directly obtained on the MSB sold medium with the combination of IBA and KT.

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通过石蜡切片观察荔枝、龙眼胚性愈伤组织的结构和胚性细胞分裂生长的方式。Cytohistological observations of embryogenic callus of litchi and longan were carried out by paraffin slice.

而非胚性愈伤组织质地松散有水渍状、表面粗糙、多呈白色。On the contrary, the non embryogenic callus was loose in texture, rough in surface, watery and white in color.

结果表明，非胚性愈伤组织和胚性愈伤组织在表面结构上存在着明显的差异。The results showed that the structure differences between embryogenic callus and non-embryogenic callus were distinct.

小麦成熟胚愈伤组织的诱导率、生长率及胚性愈伤组织发生率的基因型效应有显著差别。The effect of genotype on the rate of induction, growth and embryogenic callus initiating were different significantly.

龙眼胚性愈伤组织中内源激素、多胺含量比非胚性愈伤组织高。The levels of endogenous phytohormones and polyamines in embryogenic calli were higher than those in nonembryogenic calli.

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以石刁柏嫩茎为材料，研究了2，4-D对其初始愈伤组织诱导和胚性愈伤组织发生的影响。We studied effect of 2,4-D on initial callus inducing and embryogenic callus occurring from Asparagus tender stem culture.

本研究以大叶秦艽幼苗叶片为材料，对其进行了离体组织培养研究。Using the leaves of Gentiana macrophylla Pall, as explants, the embryogenic calli were induced and regenerated plants were produced.

结果显示，内源ABA含量的升高与胚性细胞的启动和发育密切相关，外源ABA对体细胞胚的发生也有促进作用。The results showed that the increase in endogenous ABA contents were closely related to starting and development of embryogenic cell.

二是先诱导获得愈伤组织，再经继代培养获得胚胎发生。Embryogenic callus and somatic embryos could be directly obtained on the medium only with ZT, but it was influenced by explant sources.

结果还发现胚性愈伤组织诱导培养基和胚性愈伤组织分化培养基的组合对成苗率有明显的影响作用。The combination of embryogenic callus induction and differentiation base medium had a large effect on embryogenic callus differentiation.

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通过对2，4-D浓度及生长发育时间的控制获得了高度同步化的各发育阶段体胚材料。Synchronized embryogenic cultures at different developmental stages were obtained by controlling 2,4-D concentration and developmental time.

以红姜茎尖薄片胚性愈伤组织为材料建立了胚性细胞悬浮系，并获得了再生植株。Regenerated plantlets were obtained in embryogenic cell suspension culture from embryogenic callus of stem slice in Hedychium coccineum Buch-Ham.

用透射电镜技术研究了水稻胚性悬浮细胞玻璃化冻存过程中细胞超微结构的变化规律。Ultrastructural changes in rice embryogenic suspension cells cryopreserved by vitrification were assessed using transmission electron microscopy.