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筛选共得到一株阳性杂交瘤细胞，命名为4D11。At last, we got one positive hybridoma cells named 4D11.

杂种细胞的分泌能力较强。It suggests that the hybridoma has a stronger secretory ability.

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杂交瘤细胞的大量培养是一项迅速发展的技术。Large scale culture of hybridoma cells is a fast growing technology.

是研究筛选抗IBDV单抗杂交瘤细胞的可靠方法。This is a reliable method to select the hybridoma cells for McAb of IBDV.

目的从分泌抗体的胰腺癌杂交瘤YPC3细胞扩增单抗重链可变区基因。Objective To amplify the heavy chain variable gene from a hybridoma YPC3 cell line.

高浓度IL-5可以从T细胞杂交瘤培养的上清液中获得。High concentration of IL-5 can be purified from the supernatant of T cell hybridoma.

对所得阳性杂交瘤细胞作染色体分析并对单克隆抗体进行特性分析。The chromosome of the positive hybridoma and the character of the mAb were analysed.

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方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.

实验所采用杂交瘤技术能够成功的制备环孢素A单克隆抗体。The hybridoma technique was able to prepare monoclonal antibody of Cyclosporine A successfully.

通过试管法和玻片法检测抗A抗体的效价和亲和力。Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.

目的探讨在建立了分泌抗鼠疫F1抗原的单克隆抗体杂交瘤株后，对其进行克隆化的实验方法。Objective To found a hybridoma cell cloning method when we established strains of hybridoma cells to F1 antigen.

用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.

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杂交瘤技术原理和操作过程包含了其许多优点，特异性强、方便、敏感和快速。The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.

取杂交瘤细胞株2C3腹水单克隆抗体，通过对酸碱度等五个影响因子的研究建立了最佳ELISA检测体系。The hybridoma cell 2C3 was selected to be used for further study. By the study of the pH etc, five impact factors, a Ci-ELISA established.

用活化人B细胞株3D5细胞免疫小鼠和作为筛选的靶细胞，我们建立了产生单克隆抗体5C5的杂交瘤细胞株。Using a human activated B cell line 3D5 in immunization of mouse and as targets in screening, a hybridoma producing McAb 5C5 has been established.

目的观察自制细胞扩散匣的免疫隔离作用及匣内异基因瘤细胞的在体生长情况。Objective To determine the effect of diffusion chamber for immune isolation and the proliferation of allogenic hybridoma within diffusion chamber in mice.

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另外，发现高浓度囊素能诱导杂交瘤细胞和牛睾丸原代细胞凋亡，而对鸡胚成纤维细胞无影响，囊素这种诱导细胞凋亡的生物学活性尚未见报道。The model was simple and stable. Bursin of high concentration could induce hybridoma cells and calf testis cells apoptosis, while it had no effect on CEF.

在生物反应器的批培养过程中考察了葡萄糖及谷氨酰胺浓度对杂交瘤细胞生长及代谢的影响。The effect of glucose and glutamine concentrations on the growth and metabolism of hybridoma cells was investigated in a 2 L bioreactor with batch cultures.

结论已获得稳定分泌抗恶性疟原虫复合重组抗原单克隆抗体的杂交瘤细胞株。Conclusion The hybridoma cell lines which secreted stable monoclonal antibodies against hybrid recombinant antigen of Plasmodium falciparum have been obtained.