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目的分离获得高产量、高比活性的胶原酶。Objective To isolate and purify collagenase with high quantity and activity.

还能抑制胶原酶的表达和糖化作用，从而具有抗皱纹的功效。And it also has anti-wrinkle effect by inhibiting collagenase expression and glycation.

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研究胶原酶预防肌腱鞘内粘连的作用。To investigate the mechanism of collagenase in preventing adhesion of intrathecal tendon.

结论肝胰管内灌注胶原酶消化法是一种好的消化方法。Conclusion Hepatopancreatic duct perfusing with collagenase is a good method for digestion.

冷冻干燥法制备注射用胶原蛋白酶免疫结合物。The immunizing conjugation product of collagenase in this program was prepared by EDCI method.

目的探讨胶原酶对部分椎间盘突出病人治疗无效的原因。Objective To discuss why the Collagenase cure the intervertebral disc disease but not effective.

结论通过改良并优化溶组织梭菌培养条件，可以获得低成本、高产量、高活性的胶原酶。Conclusion The collagenase with high quantity and activity could be induced in the improved medium.

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目的通过动物实验探讨胶原酶溶盘术后早期出现神经根病损原因。Objective To investigate the causes of the initial nerve roots lesion after collagenase chemonucleolysis.

后续实验还发现芍药、川芎提取物也具有胶原酶抑制效果。Further studies found Paeonia lactiflora & Ligusticum chuanxing extraction also have collagenase inhibition.

胶原酶法分离猪卵巢卵子，热溶解制备透明带抗原。Porcine oocytes were separated by method of collagenase and the antigen of zona pellucida was prepared by heating.

结论对于颈椎间盘突出症，胶原酶颈椎间盘化学松解术是一种有效的治疗方法。Conclusion With respect to herniated cervical disc, chemonucleolysis with intradisc collagenase is an effective method.

方法采用原位胶原酶循环肝灌注法分离中国实验用小型猪肝细胞，海藻酸钠-氯化钡法微囊化。Methods In situ recirculating collagenase perfusion method was used to isolate hepatocytes of Chinese experiment minipigs.

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探讨胶原酶在作用时间内是否会引起腰椎间盘髓核组织中蛋白多糖的水解。To investigate whether collagenase can cause proteoglycan hydrolization in lumbar nucleus pulposus within the action time.

就微生物胶原酶的主要产生菌来源、微生物胶原酶基因及其多样性的机制、应用等作综合阐述。The paper covers the collagenase-secreting microbes, collagenase genes and their diversity, and application of collagenase.

胶原酶是一种特异降解胶原螺旋区或明胶，而不作用于其它蛋白质底物的酶类。Collagenase is one kind of enzymes that can degrade native helical collagen fibrils or glutin but not degrade others proteins.

实验显示五倍子水提取物能显著抑制胶原酶的活性，其作用呈浓度依赖性。The experiments showed that the gallnut extract had the significant dose-dependent inhibitory effect on the activity of collagenase.

目的研究血清胶原酶含量在妊娠及分娩中的变化与宫颈成熟的关系。Objective To study the changes of serum collagenase in the pregnancy and parturition periods and its relation with cervical ripening.

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目的探讨CT引导联合靶注射胶原酶定点髓核溶解术的临床应用价值。Objective To study the clinical significance of the combined target-injection of collagenase by CT guided for local chemonucleolysis.

目的研究臭氧髓核氧化术结合胶原酶溶解术治疗腰椎间盘突出症的临床疗效。Objective To assess the therapeutic effect of oxygen-ozone combined collagenase injection for the treatment of lumbar disc herniation.

为寻找一种可以用于制革过程分析，快速、准确、简便的胶原酶活性测定方法提供参考。It is useful reference for building a fast, accurate and simple determination method for collagenase activity used in leather analysis.