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提取的粗提物经凝胶过滤后得到抗菌肽。AMP was obtained by sephadex gel filtration.
目的考察用葡聚糖凝胶法测定脂质体包封率的影响因素。OBJECTIVE To determine the trap efficiency of liposomes using Sephadex.
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方法用硅胶和凝胶柱色谱进行分离,根据理化性质、波谱特征鉴定结构。METHOD S The column chromatography of silica gel and sephadex were used to isolate those constituents.
其次,利用改性葡聚糖凝胶对低聚糖溶液进行了浓缩分离。Secondly, the paper use the modified sephadex gel to concentrate the mother liquor ofoligosaccharides.
方法采用凝胶过滤层析技术对针刺全血清进行分段分离,并将分离的血清经冷冻干燥制成干粉。Method The acupuncture serum were separated by gel filtration with Sephadex G 75 and molecular standards.
作为固定相载体,来分离蓝色葡聚糖—2000和溴酚蓝。In this experiment, we use Sephadex G-25 as phase vector to separate Blue Sephadex-2000 and bromophenol blue.
方法用硅胶、凝胶柱色谱及高效制备液相色谱等方法分离化合物,用波谱方法鉴定其结构。Methods Silica gel column, Sephadex LH-20, and HPLC were used to separate and purify the chemical constituents.
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采用离子交换和凝胶过滤分离手段,从鹅血清中分离纯化出转铁蛋白。The goose plasma transferrin was separated from goose blood by ion-exchange on DEAE-52 and sephadex gel on G-100.
目的建立葡聚糖凝胶色谱法测定头孢替坦二钠中聚合物的方法。To set up a sephadex column chromatography method for the determination of polymer impurity in cefotetan disodium.
目的考察用葡聚糖凝胶法测定脂质体包封率的影响因素。OBJECTIVE To determine the trap efficiency of liposomes using Sephadex. METHOD Tegafur liposomes was used as the model drug.
结果葡聚糖凝胶柱径高比、洗脱流速、上样量都影响脂质体与游离药物分离效果。RESULTS The study showed that the Sephadex column, the flow and the sampling amount influenced the separating of free drug from liposomes.
采用了凝胶过滤结合硫酸铵沉淀法从异常复杂的胆汁中成功分离出一种蛋白质。Biliary protein was isolated and purified by ammonium sulfate fraction and Sephadex G-75 gel filtration column chromatograph from complex pig bile.
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采用硅胶、聚酰胺、葡聚糖凝胶LH-20柱层析法和制备薄层层析法对乙酸乙酯和正丁醇提取物进行了分离、纯化。The ethyl acetate and n-butanol extraction were chromatographed on silica gel column, polyamide column, sephadex LH-20 column and preparing TLC, respectively.
本文简要地介绍了交联葡聚糖凝胶的制备和性质,对其吸附性能及在化学领域的应用进行了概述。The preparation and property of Sephadex gel were briefly introduced, the adsorption behavior and application in chemistry realm of Sephadex gel were reviewed.
方法采用硅胶柱色谱、凝胶柱色谱进行分离,通过理化性质和波谱分析方法鉴定化合物结构。Methods A new compound was isolated by the silica gel and Sephadex LH-20 column chromatograph, and identified by the physicochemical characters and spectroscopic analysis.
分别采用葡聚糖凝胶过滤法、透析法、超速冷冻离心法分离脂质体和游离药物,计算脂质体包封率。The liposomes and free drug were separated by Sephadex filtration, dialysis and refrigerated ultracentrifugation, respectively, then the entrapment efficiency of the liposomes was calculated.
将含量较高的前二者进一步纯化,乙酸纤维膜电泳和葡聚糖凝胶G-75柱层析的结果表明纯化后的褐藻酸钠、褐藻糖胶是均一的。Sodium alginate and fucoidin were further purified, the purity of which was demonstrated homogeneously by chromatography on a sephadex G-75 column and acetate cellulose membrane electrophoresis.
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方法基于葡聚糖凝胶CM-C25对维生素B6的选择性吸附,采用液滴荧光分析方法对维生素B2和B6混合溶液进行检测。Methods Based on the selective sorption of Sephadex CM-C25 resin for vitamin B6, a renewable drop spectrofluorimetry was used for simultaneous determination of vitamin B2 and B6 in a mixed solution.