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目的研究半边旗提取物5F对微管蛋白聚合的影响。Aim To study the effect of 5F on tubulin polymerization.

并对目标化合物进行了抗肿瘤体外试验和微管蛋白聚合试验。All the target compounds were tested in a tubulin assembly assay.

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这类药物和细胞分裂有关的微管蛋白结合后可抑制肿瘤细胞的生长。These drugs block cancer growth by binding to the tubulin proteins needed for cell division.

结果表明雌性激素具有显著的促进成年鼠脑微管蛋白合成的作用。Estrogen plays a much stronger role than that of testosterone in stimulating tubulin synthesis.

事实上与细胞有丝分裂密切相关的微管蛋白几乎普遍存在于所有真核细胞中。Tubulin is present in virtually all eucaryotic cells and has a strong relation with cellular mitosis.

这些不同类型的微管蛋白非常相似，并且它们能可逆性聚合成微管。The different forms of tubulin are very similar, and they can co-polymerize into microtubules reversibly.

它的作用机理独特，通过诱导和促进微管蛋白聚合形成稳定结构，从而抑制微管解聚。It possesses a unique mechanism of action as promoters of tubulin aseembly and inhibitors of microtubule disassembly.

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另外有报道色烯类衍生物可以强烈的抑制微管蛋白聚合，从而发挥抗癌作用。Another reported Chromene derivatives can strongly inhibit tubulin aggregation and play an efficient role against cancer.

作为一种抗有丝分裂药物，它能够抑制微管聚合并诱导自缔合的微管蛋白形成盘绕的螺旋形聚合体。As an antimitotic drug it can inhibit microtubule assembly and induce tubulin self-association into coiled spiral aggregates.

结论5F能抑制体外微管蛋白聚合，从而可能是影响细胞的细胞周期及诱导凋亡途径之一。Conclusion 5F inhibits tubulin polymerization and depolymerization, and leads to cell cycle arrested, and possibly induces apoptosis.

药根碱和小檗碱可能通过阻碍线粒体内信号传导通路或抑制微管蛋白的聚合诱导细胞凋亡。Jatrorrhizine and berberine may be blocking signal transduction pathways in mitochondria or inhibit the tubulin polymerization induced apoptosis.

长春花生物碱作为一类抗有丝分裂药物，能够抑制微管聚合并诱导聚合的微管蛋白解聚。As a kind of antimitotic drugs, Vinca alkaloids can inhibit microtubule assembly and induce tubulin self-association into coiled spiral aggregates.

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结果5F能抑制反应体系吸光值升高，提示它对微管蛋白聚合具有抑制作用并能抑制其解聚。Results 5F decreased the Absorption values of the reaction system. and markedly inhibited not only tubulin polymerization but also tubulin depolymerization.

研究表明，TBZ可能通过抑制微管蛋白聚合来影响染色体正常分离，诱发非整倍体。The result suggested that TBZ may induce aneuploidy by the pathway of tubulin polymerization inhibition which is able to lead to chromosomal malsegregation.

主轴微管动态增长和收缩，通过增加和微管蛋白二聚体，分别都被清除，这种特性被称为‘动力不稳定性'。Spindle micro- tubules dynamically grow and shrink through the addition and removal of tubulin dimers, respectively, a property referred to as 'dynamic instability'.

用血清诱导分化为大量表达微管组合蛋白2阳性的神经元和胶质纤维酸性蛋白阳性的神经胶质细胞。Serum-induced differentiated cells took on the shapes of neuron and astrocyte cell and were neuron tubulin 2 and glial fibrillary acidic protein positive respectively.

体外兔脑微管蛋白聚合-解聚分析表明，药根碱和小檗碱均能抑制微管蛋白的聚合，且药根碱作用强于小檗碱。Rabbit brain in vitro tubulin polymerization-depolymerization showed that jatrorrhizine and berberine can inhibit tubulin polymerization, and jatrorrhizine are stronger than that of berberine.

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紫杉醇具有聚合和稳定微管的作用，致使快速分裂的肿瘤细胞在有丝分裂阶段被牢牢固定，使癌细胞复制受阻断而死亡。Taxol is tubulin polymerization and stability in the role of the split caused rapid tumor cells in the mitotic phase was firmly fixed so that the cancer cells by blocking reproduction and death.