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什麽是连接酶的作用在脱氧核糖核酸复制的？What is the function of ligase in DNA replication?

由NEU所诱发的染色体初级断裂不能为连接酶所修复。Thus, the NEU-induced primary chromosome breakage may not be repaired by the ligase.

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CHIP属于连接酶类，具有E3泛素连接酶活性，参与能量代谢途径和新陈代谢。CHIP, an E3 ubiquitin ligase and cochaperones, involves in energy metabolism and protein quality control.

人Pirh2蛋白是一个新发现的泛素蛋白连接酶E3，能促进p53蛋白的泛素化降解。Pirh2 , a recently identified ubiquitin-protein ligase E3, can promote p53 degradation via ubiquitination.

用该方法进行T_4-RNA连接酶制备过程的活力测定表明，该方法适合于纯酶制剂的活力测定也适合于粗酶制剂的活力测定。Observations made during the purification of T_4-RNA ligase show that this method is suitable for both the pure enzyme and the crude enzyme.

由磷酸化影响的调控步骤可能与E3泛素连接酶对底物的识别有关，也可能与实际的交联反应有关。The regulatory step affected by phosphorylation could involve either recognition of the substrate by an E3 ubiquitin ligase or the actual conjugation reaction.

当聚合酶将一个核苷酸加在新互补链的起始引子之后，或接合酶认定某段核苷酸链与原始模版配对，就可利用这些反应来得出原始模版的序列。As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template's sequence is revealed.

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“L1连接酶似乎使用了与存在于自然界的核酶和蛋白酶相似的过渡态稳定策略和酸碱催化。”他说。"The L1 ligase appears to use strategies of transition-state stabilization and acid-base catalysis similar to those that exist for natural ribozymes and protein enzymes, " he said.

方法在小样本中测序检测TLR2基因中可能存在的基因多态性，再用连接酶特异检测技术在大样本进行SNP分型，并通过统计学方法分析基因多态性与结核病易感性之间相关性。Methods TLR2 gene polymorphisms were analyzed by DNA sequencing in a small amount of samples. SNPs in TLR2 gene were detected by ligase detection technique in a large amount of samples.

乳腺癌易感基因，BRCA1，编码一种E3泛素连接酶，BRCA1通过调控DNA损伤反应和DNA修复维持基因组的稳定性。The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of DNA damage response and DNA repair.

脱氧核酶具有RNA切割作用、DNA切割作用、金属鳌合作用、过氧化物酶活性、DNA激酶活性以及DNA连接酶活性等多种催化功能。Deoxyribozymes have some catalytic functions, such as RNA transesterification, DNA transesterification, porphyrin metalation, peroxidase activity, DNA kinase activity and DNA ligase activity.

乳腺癌易感基因，BRCA1，编码一种E3泛素连接酶，BRCA1通过调控DNA损伤反应和DNA修复维持基因组的稳定性。The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair.

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本文概述了植物干旱胁迫的调控机制、植物的泛素连接酶E3，并着重阐述了泛素连接酶E3介导的植物干旱胁迫反应及其作用机制。In this paper, we summarized the drought stress regulation mechanism, plant ubiquitin ligase E3, and emphatically described the ubiquitin ligase E3 response to plant drought stress and its mechanisms.