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方法采用荧光素酶报告基因方法。Methods The method of luciferase reporter gene was used.

转染72小时后测量荧光素酶的活性。Luciferase activities were measured 72 hr post-transfection.

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在体外翻译体系中加入N蛋白，体外翻译被抑制。Addition of N protein in RRL system decreased luciferase activity.

文章就荧光素酶的种类、结构及应用进行了研究。The species, structure and applications of luciferase were researched.

我们用荧光素酶报告基因的方法，探讨了这个问题。We addressed this question with the method of luciferase reporter gene.

荧光素酶的基因可以被合成并插入到生物体中或转染到细胞中。Luciferase genes can be synthesized and inserted into organisms or transfected into cells.

例如，荧光素酶可以被用于检测血库中所存血液中的红血球是否开始破裂。Luciferase can be used in blood banks to determine if red blood cells are starting to break down.

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他认为，事实上PTC124连接上荧光素酶后，“并任何迹象显示出它是一种好药。”The fact that PTC124 binds to luciferase , he says, "has nothing to do with whether it's a good drug."

本文将就生物素化荧光素酶的克隆、表达以及功能检测进行具体讨论。The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper.

其二则是虫荧光素酶，其功能实际上相当于化学反应中的催化剂。The second chemical is called a luciferase and is the substance that actually catalyzes the chemical reaction.

相对荧光素酶活性法检测GR转录激活功能的变化。The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.

我们进一步通过双荧光素酶报告系统检测了过量表达SEPT6对相关增殖通路的影响。Further, we use dual luciferase report system to detect the effect of SEPT6 on some proliferation signaling pathways.

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研究者用基因工程的大肠埃希菌细胞生产荧光素酶作为AHL浓度函数。The investigators used genetically engineered E. coli cells to produce luciferase as a function of AHL concentration.

随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.

结果经利福平处理的细胞裂解物荧光素酶比活性值与阴性对照组和溶剂组差异显著。Result The fold activation value of luciferase of rifampin group was statistically different from control group and DMSO group.

三个突变异种均显示比野生型具有更高的荧光素酶活性，并且V85I突变异种活性最强。All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity.

核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.

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相对荧光素酶活性法检测地塞米松作用后GR转录激活功能的变化。The transcriptional activation function of GR was evaluated through the detection of relative luciferase activity after dexamethasone treatment.

由于不同种类的荧光素酶的性质有一定的差异，所以本研究结果为更好的了解和利用萤火虫荧光素酶提供了一些有用的资料。Since the different kind and different function and character, what have been done in this paper would shed a light on the study of the firefly luciferase.

瞬时转染人胚胎肾细胞293T，用荧光素酶报告基因检测这两种剪切形式的转录活性。The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.