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温度明显影响原生质体融合的频率和速度。Temperature remarkably affected the frequency and the speed of protoplast fusion.

对花生与其近缘野生种间细胞融合及培养方法进行研究。The protoplast fusion and culture in peanut and its related species were studied.

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说明原生质体融合和诱变育种是筛选高效菌种的有效途径。And this shew that Protoplast Fusion and Mutagenisis were good for fungus breeding.

为嗜杀酵母原生质体融合育种提供了依据。It provides scientific basis for the breeding of protoplast fusion of killer yeast.

实验发现根霉和酵母有获得糖化力高，又能发酵产生酒香味的融合子的可能。The result showed that the protoplast of rhizopus and candida can merge each other.

研究了啤酒酵母原生质体融合株GR8的主要发酵特性。The fermentative characters of the brewer, s yeast protoplast fusant GR8 were studied.

原生质体是研究和建立真菌遗传转化系统的重要工具。Generation of fungal protoplast is an essential tool for genetic transformation system.

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纤维素酶浓度过高或过低均使产量和活力降低。It was not good for protoplast isolation at too high or low concentration of cellulase.

胚性悬浮细胞是禾谷类作物原生质体培养的理想材料。Embryogenic suspension cell is ideal material of protoplast culture of gramineous plants.

以脆壁酵母RY-3作为出发菌株，对其营养体及原生质体进行紫外线诱变处理。S. fragilis RY-3 as starter, its cell and protoplast were mutated with ultraviolet radiation.

这是迄今第一个由籼稻原生质体再生植株的报道。This is the first report of plant regeneration from the protoplast culture of an indica rice.

电镜下观察菌丝的横切面表明，处理菌丝的细胞壁消失，原生质解体。The hyphal cell wall disappeared and protoplast was dissolved by observation on electron microscope.

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本文对红麻下胚轴、试管苗茎段、子叶、真叶、腋芽及原生质体培养进行了研究。In the study, hypocotyl, internodle stem, cotyledon, leaf, axillary bud and protoplast were cultured.

通过原生质体融合和培养获得苜蓿红豆草属间体细胞杂种植株。Somatic hybrid plants between alfalfa and sainfoin were regenerated by protoplast fusion and culture.

利用双灭活原生质体融合技术获得了苏云金杆菌新菌株。The inactivated protoplast fusion method was carried out to select new Bacillus thuringiensis strains.

以粗壮假丝酵母CJ107为出发菌株，对其进行原生质体紫外诱变选育。Candida valida CJ107 was induced to get ten mutants by protoplast mutagenesis of ultraviolet irradiation.

本文研究的是一种通过原生质体融合技术构建出的细胞工程菌。In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion.

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在泰乐菌素产生菌的原生质体再生中出现有两种不同表型的菌落。Two types of colonies, big and small, appeared during the regeneration of the protoplast of Tylosin-producer.

提出了木耳原生质体释放和再生的有效方法。An effective method of protoplast liberation and protoplast reversion of Auricularia auricula was put forward.

本实验首先确定潮霉素浓度对香菇和平菇菌丝和原生质体再生的影响。Hygromycin B resistance was studied on the mycelium and protoplast of Lentinula edodes and Pleurotus ostreatus.