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于是我们开始做转座子的突变发生。So we started doing transposon mutagenesis.

提示我们危货站空气污染物具有直接遗传毒性作用。It indicated that air pollution of DCARTS had direct mutagenesis.

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结论物理诱变可以有效提高弹性蛋白酶的酶活。Conclusion Physical mutagenesis can enhance the activity of elastase.

采用驯化与紫外线诱变并用的方法研制茶酒酵母。Methods of domestication and UV mutagenesis were used to prepare tea wine yeast.

定点突变是研究基因功能的一种关键方法。Site-directed mutagenesis is one of the key methods used to study gene function.

错误突变理论引导了通过突变来控制致病病毒的策略。Error catastrophe theory has led to a strategy of mutagenesis to control disease viruses.

初步建立了SR软X射线对微生物辐射诱变的方法。The method of SR soft X-ray radiation mutagenesis on microbe was established preliminarily.

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目的介绍一种简便、快速、准确构建定点诱导突变体的新技术。Objective To introduce a new technique for rapid construction of gene site-directed mutagenesis.

在其他微生物中主要应用转座子突变和自杀载体进行基因打靶。Transposon mutagenesis and suicide vectors are mainly used for gene targeting in other microbes.

以上结果表明，空间诱变可能为食用菌的遗传育种提供新途径。The results indicated that space mutagenesis might provided a new way for edible fungus breeding.

为了发展基因突变技术，介绍一种新型的基因定点突变方法。To develop the gene mutagenesis technique, a novel method of site-directed mutagenesis was introduced.

以粗壮假丝酵母CJ107为出发菌株，对其进行原生质体紫外诱变选育。Candida valida CJ107 was induced to get ten mutants by protoplast mutagenesis of ultraviolet irradiation.

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方法采用氯化锂和紫外线复合诱变单孢子悬浮液。Methods By using complex mutagenesis of LiCl and ultraviolet rays, we deal with the simple spore suspension.

目的以小鼠骨髓PCE微核率为指标，观察三种品牌的染发剂是否具有诱变性。Objective To study the mutagenesis of three kinds of hair dye using mouse bone marrow PCE micronucleus test.

目的探讨核蛋白在镍对DNA氧化和致突变中的作用机制。Objective To study the role and mechanism of nucleoprotein in DNA oxidation and mutagenesis induced by nickel.

细胞周期分析可为研究柑橘愈伤组织理化诱变提供依据。Cell cycle analysis can provide reference for researching the physical and chemical mutagenesis to citrus calli.

构建插入突变库的方法有T-DNA插入突变、转座子插入突变和质粒介导的插入突变。Insertional mutagenesis using T-DNA, transposons and plasmids are commonly methods to creating a mutant library.

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过去主要是通过亲和标记和点突变等实验方法进行研究。In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.

结果表明某些定点突变可以揭示影响酶活的重要功能域。The results indicated that some site-directed mutagenesis can reveal important functional domains of enzyme activity.

证明C812A、C814A的突变确实能显著改变包涵体的溶解性。It demonstrated, that the mutagenesis of C812A and C814A could signally change the dissolvability of inclusion bodies.